Phlebotomy Order of Draw Practice Test 2025 - Free Phlebotomy Practice Questions and Study Guide

When preparing a site for blood culture, it is essential to use an antiseptic that effectively reduces the risk of contamination from skin flora. The choice of betadine (povidone-iodine) or a chlora prep (chlorhexidine) is particularly important in this context due to their broad-spectrum antimicrobial properties.

Betadine is an iodine-based antiseptic, and it works by killing bacteria and other pathogens on the skin. Chlorhexidine is another effective antiseptic that has residual activity, meaning it continues to kill bacteria even after initial application. Both agents significantly aid in reducing the microbial load at the site of puncture, which is crucial for obtaining a reliable culture result.

Using these antiseptics not only lowers the risk of false-positive cultures caused by contamination during the blood collection process but also adheres to the best practices in phlebotomy, ensuring patient safety and accuracy of diagnostic tests.

In contrast, while alcohol swabs can disinfect the skin to some extent, they do not provide the same level of antimicrobial effectiveness as betadine or chlorhexidine, especially for blood cultures. Cleaning with soap and water is beneficial for general hygiene but lacks the necessary sterilizing action needed in a clinical setting for blood culture preparation. Hence,